
Chapter 2 Microscopy
17
Set the microscope for the bright-field microscopy under the episcopic illumination.
If the current unit is the one shown in the column at the right edge under “Other items required” in Table 2.1,
pull out each item from the optical path.
1 Push in the optical path changeover lever for the
eyepiece tube and select 100% for the binocular
eyepiece. (See 3.4.1.)
2 Push in the eyepiece tube/back port changeover
lever to select 100% for the eyepiece tube. (See
3.4.1.)
3 Push in the BD field changeover lever to select the
“BF (bright-field)” position. (See 3.3.)
4 Turn the revolving nosepiece to place the 10X
objective into the optical path. (See 3.9.2)
The nosepiece addresses (1 to 7) on the
front-display light up to indicate the position of the
revolving nosepiece. (See 3.9.3.)
5 Place the NCB11 filter in the filter turret into the
optical path and compensate color temperature.
(See 3.10)
6 Adjust the brightness roughly with the brightness
control dial and the desired ND filter in the filter
turret. (See 3.2.1 and 3.10.)
7 Turn the field diaphragm dial and the aperture
diaphragm dial counter-clockwise to the limit to
fully open the field diaphragm and the aperture
diaphragm for the episcopic illumination. (See 3.11
and 3.12.)
Place the sample onto the stage and adjust the focus and the brightness.
1 Use the sample holder according to the selected
sample. Place the sample onto the stage and
move the stage using the stage movement knob
for the X/Y direction so that the observation
position comes to the center of the view-field. (See
3.8.)
2 Turn the coarse/fine focus knobs and focus on the
target. (See 3.7.)
3 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
Adjust the diopter and the interpupillary distance.
1 Adjust the interpupillary distance. (See 3.5.)
2 Adjust the diopter. (See 3.6.)
2-1
2-5, 6
2-4
2-6
2-4
2-3
2-7
3-2
3-3
3-1
3-1
2-2
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